不同培养条件对互叶白千层丛生芽增殖的影响

The Influence of Different Culture Conditions on the Multiple Shoot Multiplication of Melaleuca leucadendron

[目的]研究互叶白千层丛生芽增殖的最优条件。[方法]研究不同基本培养基、碳源及其质量浓度、激素组合对互叶白千层丛生芽增殖的影响。[结果]MS为较好的基本培养基,白糖为较适碳源,白糖的适宜浓度为40 g/L,较好的激素组合是6-BA 0.8 mg/L+2,4-D 0.01 mg/L。[结论]丛生芽的增殖效果与基本培养基有关;一定浓度范围内,增殖倍数随着碳源浓度的增大而增大;低浓度的2,4-D更有利于丛生芽的增殖,配合较低浓度的6-BA效果更好。

[Objective]The aim was to find better culture conditions on multiple shoot multiplication for Melaleuca leucadendron.[Method] The impact of different basic medium,different carbon source and concentration,different hormone combination on multiple shoot multiplication was studied.[Result]The results showed that MS was better basic medium,40 g/L white suger was better carbon source,6-BA 0.8 mg/L＋2,4-D0.01 mg/L was better hormone combination.[Conclusion]Multiple shoot multiplication effect related to basic medium.In a certain concentration range,the proliferation times increased with the increase of carbon concentration.Low concentration 2,4-D with low concentration 6-BA was beneficial to multiple shoot multiplication.