西藏黄牛胎儿成纤维细胞的分离与培养

Isolation and Culture of Fetal Fibroblasts of Tibetan Yellow Cattle

[目的]以西藏黄牛胎牛为材料,建立西藏黄牛胎儿成纤维细胞系,为在西藏特殊生境条件下高原物种的动物克隆及其他细胞生物学研究提供理想的生物学材料。[方法]对西藏黄牛胎儿成纤维细胞的分离和培养进行了研究。利用组织块法成功分离培养西藏黄牛胎儿成纤维细胞,对细胞的传代培养、形态学和动力学进行观察与分析。[结果]所建立的西藏黄牛胎儿成纤维细胞系具有典型的成纤维细胞形态;西藏黄牛胎儿成纤维细胞要经历滞留期、对数期、平台期,分别为0-2 d、2-7 d、7-8 d,细胞群体倍增时期为60 h。[结论]成功培养了西藏黄牛胎儿成纤维细胞。

[Objective] To isolate fetal fibroblasts from Tibetan yellow cattle and thus to provide the ideal biological material for cloning and cellular biology of animals inhabiting in the Tibet Plateau.[Method] The fibroblast cells were isolated from Tibetan yellow fetus by explant culture method.The subculture,morphology and dynamics were observed.[Result] The fetal fibroblasts of Tibetan yellow cattle had typical morphology of fibroblast cells.The isolated cells had three growth stages including retention period（0-2 d）,logarithmic phase（2-7 d） and platform phase（7-8 d）.Its doubling time was 60 h.[Conclusion] Fetal fibroblasts of Tibetan yellow cattle have been successfully cultured.