新型固定化酵母细胞发酵生产乙醇的研究

The Research of Newly Immobilized Yeast's Fermentation of Ethanol

[目的]说明利用甘蔗块作为酵母固定化材料进行固定化酵母发酵相对于游离酵母发酵来说具有优越性,同时探究利用甘蔗汁和废糖蜜作为发酵培养基分别进行发酵时的发酵效果。[方法]共设计12个发酵组,研究发酵培养基和是否进行固定对发酵效果的影响。[结果]以甘蔗汁为发酵培养基时固定化酵母发酵液中乙醇平均体积分数比游离酵母发酵高0.7%,以废糖蜜为发酵培养基时固定化酵母发酵液中乙醇平均体积分数比游离酵母发酵高0.76%。甘蔗汁培养基与废糖蜜培养基对总体发酵效果的影响非常接近。[结论]综合考虑甘蔗汁与废糖蜜的成本,废糖蜜是工业发酵生产乙醇用培养基的更优选择。

[Objective]The experiment was designed to prove that using sugarcane clump as the immobilizing material to ferment was better than dissociative yeasts＇ fermentation,and at the same time to explore the fermentation effect of sugarcane and blackstrap when they were used as the fermentation culture.[Method] 12 fermentation groups were arrange to study the influence of the fermentation culture and immobilizing or not to fermentation effect.[Result]The fermentation result showed that when blackstrap was used as the fermentation culture,the average volume fraction of ethanol of the immobilized yeast in the fermentation liquor was 0.76% more than dissociative yeasts＇ fermentation;and when sugarcane juice was used as the fermentation culture,the average volume fraction of ethanol of the immobilized yeast in the fermentation liquor was 0.7% more than dissociative yeasts＇ fermentation.On the other hand,the influence of sugarcane juice and blackstrap to fermentation was almost the same.[Conclusion] Considering the cost of sugarcane juice and blackstrap integrally,blackstrap was the better choice in industrial production of ethanol.