陕南蔬菜根结线虫群体的mtDNA-PCR-RFLP快速种类鉴定

Species Identification of Root-knot Nematodes on Vegetables in South Shaanxi Employing mtDNA-PCR-RFLP

[目的]运用mtDNA-PCR-RFLP技术快速鉴定陕南蔬菜根结线虫,为根结线虫的有效防治提供参考。[方法]在形态学、同工酶鉴定的基础上,运用mtDNA-PCR-RFLP电泳技术,对采自陕南3市18县区的37个为害蔬菜的根结线虫种群进行了鉴定。[结果]在所鉴定的种群中,32个种群为南方根结线虫(Meloidogyne incognita),2个种群为花生根结线虫(M.arenaria),1个种群为北方根结线虫(M.hapla),1个种群为南方根结线虫和花生根结线虫的混合群体,1个种群为南方根结线虫和北方根结线虫的混合群体;其中南方根结线虫是陕南为害蔬菜的主要根结线虫。[结论]形态学方法应仅作为辅助鉴定手段,而同工酶表型和mtDNA-PCR-RFLP分析均可作为快速而可靠地鉴定中国常见根结线虫种类的方法。

[Objective] In order to provide reference for the effective control of root-knot nematodes,the root-knot nematodes on vegetables in South Shaanxi were identified rapidly by mtDNA-PCR-RFLP.[Method] Thirty-seven root-knot nematode populations on vegetables collected from South Shaanxi were identified based on morphology,isozyme phenotypes and mtDNA-PCR-RFLP analysis.[Result] 32 populations were identified as Meloidogyne incognita,2 populations as M.arenaria,1 populations as M.hapla,1 population as a mixture of M.incognita and M.arenaria and 1 population as a mixture of M.incognita and M.hapla.M.incognita was the predominant species in South Shaanxi.[Conclusion] Morphological identification methods should be used only as a supplementary method,while isozyme phenotypes and mtDNA-PCR-RFLP analysis were rapid and reliable methods for identification of root-knot nematode common species in China.