龙爪槐提取物对铜绿微囊藻生长的抑制作用研究

Study on the Inhibitory Effect of Sophora japonica Extracts against the Growth of Microcystis aeruginosa

[目的]探讨龙爪槐提取物对铜绿微囊藻生长的抑制作用。[方法]测定了龙爪槐叶片浸取液对铜绿微囊藻的增殖抑制效果,并对活性组分进行了初步研究和分析。[结果]龙爪槐叶片的无水乙醇浸提物经正己烷、乙酸乙酯、正丁醇和水依次分配后,提取物主要成分为极性组分(60%以上),而含量约为25%的非极性组分(正己烷相)具有显著的抑制铜绿微囊藻生长的效果。在25和50 mg/L正己烷相组分作用下铜绿微囊藻的7 d生长抑制率分别达75%和90%以上,同时还伴随着细胞中叶绿素a的破坏。[结论]为防治微囊藻水华提供了理论依据。

[Objective] The research aimed to discuss the inhibitory effect of Sophora japonica extracts against the growth of Microcystis aeruginosa.[Method] The inhibitory effect of extracted liquid of Sophora japonica leaf against the growth of Microcystis aeruginosa was measured.Moreover,the active component was studied and analyzed initially.[Result] The absolute alcohol extract of Sophora japonica leaf was separated to n-hexane,ethyl acetate,n-butanol and water phases in turn.The polar fractions were found being the majority（60%）.The non-polar fraction in n-hexane（about 25%） was found significantly inhibiting the growth of M.aeruginosa.The inhibition rates of fraction in n-hexane at the concentrations of 25 and 50 mg/L against M.aeruginosa in 7 d were higher than 75% and 90% respectively.In addition,chlorophyll a of M.aeruginosa was also destroyed in the presence of the hexane fraction.[Conclusion] The research provided the theory basis for preventing and controlling the water bloom of M.aeruginosa.