超声波法提取香蕉皮多糖的条件优化及其生物活性

Optimization of Extraction for Banana Peel Polysaccharides and Its Bioactivity

[目的]优选香蕉皮多糖的提取工艺条件及多糖生物活性研究。[方法]运用超声波技术提取香蕉皮多糖,考察料液比、水提时间、水提温度和提取次数等因素对提取工艺的影响,用苯酚-硫酸法测得香蕉皮中多糖含量。利用MTT法检测多糖对人乳癌MCF_7细胞增殖的影响。[结果]香蕉皮多糖提取的最佳条件为:超声时间210 s,超声功率320W,料液比1∶50,提取温度为50℃,水提时间1h,提取2次。[结论]利用超声波法提取香蕉皮中的多糖,提取效率高,工艺稳定可靠,方法简便可行;香蕉皮多糖对人乳MCF_7细胞有明显的抑制增殖作用。

[Objective]To optimize the best extraction conditions of polysaccharides from dried banana peel and to investigate its bioactivity.[Method]Base on the single factorexperiments,factors such as material to solvent ratio,extracting time,extraction temperature and extractiom times were optimized by ultrasonic assistant,and the phenol-sulfuric reaction was used for the determination of polysaccharide.MTT assay was used to study the effect of banana peel polysaccharides on breast cancer MCF-7 cells.[Result]The optimum process conditions for polysaccharides were：ultrasonic power 320 W,ultrasonic time 210 s,extraction temperature 40 ℃ and solidtosolvent ratio 1∶ 50 andextracting fouir times.[Conclusion]Ultrasonic extraction of polysaccharides from banana peel is efficient and simple,the method is stableand reliable.The banana peel polysaccharide can inhibit the proliferation of breast cancer MCF-7 cells.