PR预处理对急性STEMI再灌注心肌HIF-1α表达的实验研究

Experimental Research on HIF-1α Expression in Propofol Preconditioning on Acute STEMI Reperfusion Myocardial

目的:探索丙泊酚(propofol,PR)在急性ST段抬高型心肌梗死再灌注损伤的作用机制,研究丙泊酚对急性STEMI再灌注过程中缺氧诱导因子-1α(hypoxia inducible factor 1α,HIF-1α)表达的影响。方法:60只SD大鼠,雌雄不限,随机分为四组:①假手术对照组(A组):开胸但不结扎;②I/R模型组(B组):缺血前10min开始持续静脉滴注以生理盐水稀释的脂肪乳1.0ml·kg-1,至再灌注持续2h结束;③丙泊酚Ⅰ组(C组):丙泊酚6mg·kg-1稀释于生理盐水,缺血前10min开始持续静脉滴注,至再灌注持续2h结束;④丙泊酚Ⅱ组(D组):丙泊酚12mg·kg-1稀释于生理盐水,缺血前10min开始分别持续静脉滴注,至再灌注持续2h结束。实验12h后取出心脏组织。通过WesternBlot方法检测再灌注心肌组织HIF-1α的表达;用RealTimePCR方法检测HIF-1αmRNA的表达。结果:与假手术组比较,HIF-1α活化,mRNA表达量也显著增加(P<0.05),HIF-1α的表达量也明显高于I/组(P<0.05)。结论:丙泊酚可能通过上调HIF-1α的表达,从而保护急性STEMI再灌注损伤心肌。

Objective：To investigate the effect of propofol on cardiac myocyte apoptosis and the expression of hypox iainducible factor 1α（HIF-1α） following acute STEMI reperfusion myocardial in rats.Methods：Sixty SD rats were randomly allocated into four groups with 15 rats each：control group（A）,ischemia/reperfusion group（B）,6 mg·kg-1 propofol group（C）,12 mg·kg-1 propofol group（D）.The acute STEMI reperfusion myocardial were identified by Western Blot and Real Time PCR（RT-PCR）.Results：The expression of HIF-1αlower in group B than in group C and group D,but higher than those in group A.The expression of HIF-1αlower in group C those in group D,but higher than those in group B.Conclusion：Propofol can protect acute STEMI reperfusion myocardial significantly,which may be related to increase in expression of HIF-1αin the acute STEMI reperfusion myocardial.