脂筏微区超分子聚集体结构的稳定性

Stabilities of Supramolecular Complex Structures in Lipid Rafts Microdomains

脂筏微区结构的动态特征与稳定性决定着细胞膜的功能.脂筏在体分离形态观测呈现出烧瓶状凹陷、球状及椭球状结构.体外模拟实验表明,鞘磷脂(SM)/胆固醇(Chol)/1,2-二油酸甘油-3-磷脂酰胆碱(DOPC)呈现出明显的液态有序相和液态无序相共存的相分离膜结构.1,2-二油酸甘油-3-磷脂酰乙醇胺(DOPE)和神经酰胺(Ceramide)促使SM/Chol液态有序相发生重排.单层膜分析表明过量分子面积决定分子间的相互作用,过量吉布斯自由能决定膜的稳定性.通过动物细胞提取脂筏与体外模拟脂筏相结合的方法,从超分子水平阐述了脂筏微区结构与功能的生物学意义,为生物膜的研究提供了理论依据和实验支持.

The dynamic and stability characteristics of lipid raft microdomains dominate the functions of cell membranes.The flask-shaped caveolae,spherical and ellipsoidal structure of lipid rafts extracted from animal cells have been observed.The results of simulated experiments showed that the films of SM/Chol/DOPC took on obvious liquid ordered and liquid disordered coexisted phase.It could be induced to rearrangement by DOPE and ceramide on SM/Chol phase.In the mixed monolayers,the excess molecular area and excess Gibbs free energy determines the molecular interactions and stability respectively. The biological significance and the relationship between structure and function lipid rafts were explored on super-molecular level by combining extraction from cells in vivo and simulated in vitro method. This project conduction can provide theoretical evidence and experimental support for studying biomembrane.