Wistar大鼠重度失血性休克模型制备影响因素的研究

Factors that affect the preparation of a severe hemorrhagic shock model of Wistar rats

目的探讨Wistar大鼠重度失血性休克模型制备的影响因素。方法 70只雄性Wistar大鼠,体质量为180～220 g,麻醉后经股动脉放血40%制备重度失血性休克模型,对放血后存活大鼠动脉血中pH、PaO2、PaCO2三项指标进行正态性分析,获得其分布规律,随后计算95%参考值范围,再将所有实验大鼠按照三项指标是否均在参考值范围内分为符合组(n=61)和不符合组(n=9),通过连续校正的2检验对两组大鼠的存活与否进行分析,最后通过多重logistic回归方法分析三项指标与存活与否的相关性。结果经统计学分析发现,两组Wistar大鼠的pH值、PaO2、PaCO2值均呈偏态分布。通过计算获得存活大鼠三项指标的95%参考值范围:pH(7.30～7.59),PaO2(73～110)mmHg,PaCO2(32～50)mmHg。符合组(80.33%)与不符合组(33.33%)存活率相比差异具有统计学意义(P<0.01),其中pH值、PaCO2的交互作用与存活相关性较高。结论在重度失血性休克模型的制备中,符合组pH(7.30～7.59),PaO2(73～110)mmHg,PaCO2(32～50)mmHg范围的Wistar大鼠相对于不符合组更易于存活,制备成功率更高。其中,pH值、PaCO2的交互作用对存活与否影响更大。

Objective To study the factors that affect the preparation of a severe hemorrhagic shock model of Wistar rats. Methods Seventy male rats were anesthesized and eannulated. Forty percent of the total blood volume was withdrawn via the arteria femoralis. Then the distribution of Wistar rats＇ pH, PaO2 and PaCOz was analyzed and the 95% reference values were calculated. The rats were divided into normal group （ n = 61 ） and abnormal group （ n = 12 ） according to the reference values. The continuity adjusted Chi-square test was used to compare the intergroup survival rates. Then multiple logistic regression analysis was used to evaluate the survival-correlated parameter. Results The distribution of pH, PaO2 and PaCO2was all skew. The 95% reference values were calculated as pH（ 7.30 -7.59 ） , PaOz （73 - 110 ）mmHg and PaCO2 （32 -50）mmHg. There was significant difference between the two groups （P 〈 0.01 ）. The survival rate of the nor- real group （80.33%） was higher than that of the abnormal group（33.33% ）. pH and PaCO2were correlated with the sur- vival rate. Conclusion The rate of success may be higher if the pH within 7, 30 -7.59,PAO2 within 73 - 110 mmHg and PaCO2within 32 -50 mmHg when the Wistar rats are used as a severe hemorrhagic shock model, pH and PaCOz are of greater significance for survival.