pp32在前列腺癌细胞中的作用研究

Effects of pp32 on prostate cancer cells

目的研究pp32在前列腺癌细胞中的作用。方法将本实验室先前保存的pcDNA3.1和pcDNA3.1-pp32质粒,转染至Pc3M细胞,构建Pc3M-pp32稳定细胞株。吉姆萨染色实验观察Pc3M细胞的形态变化,通过Transwell小室法检测细胞迁移能力。黏附测定实验鉴定细胞的黏附能力,采用MTT法测定细胞生长曲线。最后,Western印迹检测N-钙黏蛋白(N-cadherin)在细胞中的表达。结果成功筛选并获得了稳定过表达pp32的细胞株(Pc3M-pp32),pp32能引起Pc3M细胞形状的显著改变,引起人前列腺癌细胞Pc3M迁移和黏附能力的增加,明显促进Pc3M细胞的生长,并有效抑制N-钙黏蛋白在Pc3M细胞中的表达。结论过表达pp32可引起Pc3M细胞的形状改变,并可促进Pc3M细胞的迁移、黏附以及生长。

Objective To explore the effect of pp32 on prostate cancer cells. Methods The pcDNA3.1 and pcDNA3.1-pp32 plasmids previously stored in our laboratory were transferred into Pc3M cells in order to construct stable Pc3M-pp32 cell lines. Morphological changes of Pc3M cells were observed by Giemsa staining experiments, and the cells ability of migration was measured through Transwell＇s chamber assays. The cells ability of adhesion was assessed by adhe- sion experiments, and MTT assays were used to determine the cell growth curve. Finally, the expression of N-cadherin was investigated by Western blotting. Results We successfully obtained Pc3M cell lines with pp32 over-expression （ Pe3M- pp32）, and pp32 significantly induced morphological changes of Pc3M cells. Pp32 could also enhance the migration and adhesion of human prostate cancer cells Pc3M. Pp32 could obviously increase Pc3M cell growth and effectively inhibit the expression of N-cadherin in Pc3M cells. Conclusion Over-expression of pp32 leads to morphological changes of Pc3M cells, and promotes the migration, adhesion, and growth of these cells.