摘要
以牡丹皮为原料研究了丹皮酚的提取工艺,并对丹皮酚的荧光光谱性质进行了研究,探讨了酸碱对丹皮酚荧光性质的影响。研究结果表明,随着丹皮酚浓度的增加荧光强度逐渐减弱;在酸性溶液中荧光强度均随pH的降低而减弱,在碱性溶液中荧光强度均随pH的升高而减弱,甚至产生荧光猝灭。此外,丹皮酚与人血清白蛋白(HSA)结合,可导致HSA荧光猝灭。
The extraction process of paeonol was investigated with coxtex moutan as material raw,and the fluorescence properties of paeonol were studied,and the effect of acid-base on fluorescence properties of paeonol was discussed.The fluorescence intensity was lower along with increase of paeonol concentration,and that also decreased with increase of pH value in acid solution,while the in the alkaline solution fluorescence intensity decreased even fluorescence quenching with increasing of pH values.In addition,fluorescence quenching of HSA occurred in order to paeonol combined with human serum albumin(HSA).
出处
《光谱实验室》
CAS
CSCD
北大核心
2011年第5期2626-2630,共5页
Chinese Journal of Spectroscopy Laboratory
基金
河南省教育厅自然科学研究计划项目(2009B150015)
关键词
牡丹皮
丹皮酚
荧光光谱
Coxtex moutan
Paeonol
Fluorescence Spectrum