广西龙荔遗传多样性的ISSR分析

Genetic diversity analysis of Dimocarpus confinis in Guangxi by intersimple sequence repeats(ISSRs)

选用l2条ISSR引物组合分析了广西67个龙荔单株和10个龙眼品种（单株）、12个荔枝品种以及l0个野生荔枝单株的遗传多样性,均得到了清晰指纹。在所扩增的151条ISSR主带中,有127条具有多态性,多态性比率占84.11%。所用引物可将供试的品种（单株）区分开来。供试龙荔单株的遗传相似系数在0.61~0.97,表明广西龙荔种质具有较广泛的遗传多样性。龙荔与龙眼、荔枝间遗传物质存在明显的差异,99份供试材料间的相似系数变化在0.53~0.98,在相似系数0.53处荔枝供试材料自聚为1类,而龙荔与龙眼聚为1类,表明龙荔与荔枝的遗传差异较大,龙荔在遗传物质上更接近龙眼。

Twelve pairs of ISSR primer combinations were used to determine the genetic diversity of Dimocarpus confinis in Guangxi.Materials included 67 D.confinis individual plants,10 longan varieties,12 litchi varieties and 10 wild litchi plants in Guangxi and all the materials obtained clear fingerprints.A total of 151 major ISSR bands were observed,and 127 bands（84.11%） were polymorphic.All of the cultivars could be distinguished with the markers used.Genetic similarities among all D.confinis ranged from 0.61 to 0.97,and it indicated that D.confinis germplasm in Guangxi had abundant genetic diversity.There were obvious differences among D.confinis,longan and litchi,genetic similarities among all 99 taxa ranged from 0.53 to 0.98,litchi formed a single cluster and D.confinis and longan formed another cluster at Dice coefficient 0.53.The results showed that D.confinis was closer to longan in genetic material.