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CDA-Ⅱ抑制肺腺癌细胞增殖及C-erbB2表达的实验研究

Experimental study of proliferation and C-erbB2 expression of lung cancer cells induced by cell differentiation agent-Ⅱ
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摘要 目的:研究细胞分化剂CDA-Ⅱ(cell differentiation agent-Ⅱ)抑制人肺腺癌细胞A549增殖及对癌基因C-erbB2表达的影响。方法:不同浓度的CDA-Ⅱ作用人肺癌细胞A549,用CCK-8法检测细胞增殖抑制率;RT-PCR和Western blot检测经CDA-Ⅱ作用后A549细胞C-erbB2表达的改变。结果:CDA-Ⅱ能明显抑制肺腺癌细胞增殖,且抑制效应呈剂量依赖性。C-erbB2在CDA-Ⅱ抗人肺癌细胞A549增殖中表达下调。结论:CDA-Ⅱ可有效抑制人肺癌细胞A549增殖,该作用可能与抑制C-erbB2的信号传导通路有关。 AIM: To investigate the effects of CDA-Ⅱ(Uroacitide) which is a healthy human urine extraction on proliferation and C-erbB2 expression of human lung cancer cell line A549. METHODS: The effect of CDA-Ⅱ on cellular viability was determined by CCK-8 assay.The change of C-erbB2 expression in A549 cells induced by CDA-Ⅱ was detected by RT-PCR and Western blot.RESULTS:After A549 cells were treated with various concentration of CDA-Ⅱ,cell proliferation was significantly inhibited in a dose-dependent manner.The expression of C-erbB2 was downregulated in A549 cells induced by CDA-Ⅱ.CONCLUSION: CDA-Ⅱ can inhibit the growth of A549 cells associated with downregulation of C-erbB2,which indicates that C-erbB2 is involved in CDA-Ⅱ induced proliferation inhibition of A549 cells.
出处 《中国临床药理学与治疗学》 CAS CSCD 2011年第8期847-851,共5页 Chinese Journal of Clinical Pharmacology and Therapeutics
基金 广东省医学科研基金项目(B2007088) 广州医学院科学研究项目(2006ZR016)
关键词 CDA-Ⅱ 人肺癌A549细胞 增殖 C-ERBB2 CDA-Ⅱ Human lung cancer cell line A549 Proliferation C-erbB2
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