益肾化瘀方对再障大鼠的疗效及外周血5-羟色胺分泌的影响

effects of herbal medicine of reinforcing kidney and removing blood stasis on rats with aplastic anemia and its effect on secretary of periphery 5-HT

目的:观察益肾化瘀方对再障大鼠的疗效,并分析其对大鼠外周血5-羟色胺分泌的影响。方法:SD大鼠随机分为4组:空白组、模型组、中药组、中药+酮色林组(酮色林1 mg.kg-1.d-1),于给药后第0、7、12、16天分别取外周血,高效液相色谱法检测5-羟色胺的浓度,并观察各组大鼠骨髓病理组织学及T细胞亚群的变化。结果:中药组及中药+酮色林组大鼠病理组织学改变优于模型组,但仍较空白组差。中药组及中药+酮色林组其CD4+亚群较模型组高,但仍低于空白组,而CD8+亚群则低于模型组,但仍高于空白组。CD4+/CD8+比值中药组及中药+酮色林组较模型组增高,但仍低于空白组。高效液相色谱分析显示:中药组及中药+酮色林组5-羟色胺浓度在第12天较第0、7天有显著提高,提高效应持续至第16天。组间比较发现中药组和中药+酮色林组其5-羟色胺浓度在第12、16天较模型对照组和空白组高,而中药组及中药+酮色林组之间各时间点5-羟色胺浓度差异无统计学意义。结论:益肾化瘀方药能使大鼠外周血5-羟色胺浓度提升同时改善其造血功能,提示该方药治疗再障的机制可能与其促进外周5-羟色胺分泌相关。

AIM： To study the therapeutic effect of herbal medicine of reinforcing kidney and removing blood stasis on rats with aplastic anemia,and analyzed its effect on secretary of periphery 5-HT. METHODS： Male SD rats were randomized into 4 groups： blank group,model group,TCM（traditional Chinese medicine） group,TCM＋ketanserin group（ketanserin 1 mg·kg-1·d-1）.The peripheral blood of rats in each group were taken at the 0,7th,12th,16th day.The concentration of plasma 5-HT was analyzed by HPLC,at the same time T cell subsets were also analyzed in each group.RESULTS：Pathological changes in bone marrow of TCM group and TCM＋ketanserin group were better than the model group,but still worse than the blank group,CD4＋ subsets in TCM and TCM＋ketanserin group were higher than the model group,but still less than the blank group,CD8＋subsets in TCM and TCM＋ ketanserin group were less than the model group,but still higher than the blank group,The CD4＋/CD8＋ ratio in TCM and TCM＋ketanserin group were higher than the model group,but still less than the blank group.HPLC showed that the level of 5-HT of TCM and TCM＋ ketanserin group were significant elevated at 12th day compared with the 0,7th day.The effect of elevation lasts to the 16th day.The group comparison showed that the levels of 5-HT of TCM and TCM＋ ketanserin group were higher than the blank control and model group at 12th,16th day,but the difference of level of 5-HT between the TCM group and the TCM＋ketanserin group were not significant at 0,7th,12th,16th day.CONCLUSION： The therapeutic effect of herbal medicine of reinforcing kidney and removing blood stasis can promote the level of 5-HT,at the same time it can improve the function of haematogenesis.The result promotes that the mechanism of the therapeutic effect of herbal medicine of reinforcing kidney and removing blood stasis may have something to do with the promotion of secretion of 5-HT.