大鼠高同型半胱氨酸血症对抑郁的影响

Effects of Hyperhomocysteinemia （HHcy） on Depression in Rats

目的探讨高同型半胱氨酸血症（HHcy）对大鼠抑郁和动脉粥样硬化（As）的影响。方法20只Wistar雄性大鼠随机分为HHcy模型组及正常对照组，每组10只，模型组连续给予高L-蛋氨酸灌胃建立HHcy模型，在4、6、8周检测血浆总同型半胱氨酸（THcy）和“强迫游泳不动法”的动物不动时间以反映HHcy引发抑郁的情况，并通过主动脉内膜脂质斑块检测观察第8周AS情况。结果在给药的第6周，模型组动物血浆THcy（15．36±3．6gmol／L）和“强迫游泳”不动时间（193．8±27．7S）、第8周，腹主动脉内膜脂质斑块百分率（7．45％±3．99％）较正常组（分别为11．38±1．78μmol／L、113．1±27．39S和4．05％±2．22％）有显著的增高（P〈0．01或P〈0．05）。结论高同型半胱氨酸血症模型大鼠血浆THcy升高，“强迫游泳”不动时间延长，内膜脂质斑块百分率增大，表现出明显的抑郁症状和AS样改变。

Objective To study the effects of byperhomocysteinemia on depression and atherosclerosis in rats. Method Twenty male Wistar rats were randomly divided into model group and normal group （each n=10）. The rat model was established by feeding L-methionine with gastric irrigation daily. After four, six and eight weeks, the total homocysteine （THcy） concentration in plasma and the immobility time of ＂forced-swimming test＂ in five minutes were respectively detected to reflect the situation of depression caused by HHcy. Eight weeks later, the change of atherosclerosis in lipid plaque coverage was detected in abdominal aorta intima. Result After six week＇s dosing, the concentrations in plasma of the model group （15.36 ± 3.6 μmoL/L） were significantly higher than those in the normal group （11.38± 1.78 BmoL/L, P〈0.01）. All the immobility times in 4 times ＂forced-swimming test＂ were prolonged and more significantly increasing （193.8 ± 27.7 s） than the result of normal group （113.1± 27.39 s） at end of sixth week （P〈0.001）. At same time, the percentage of lipid plaque coverage in abdominal aorta intima was significantly increased at the end of the test （the model group was 7.45%± 3.99%, the normal group 4.05% ± 2.22%, P〈0.05）. Conclusion The plasma THcy concentration of model group was higher than that of normal group. The rats that had hyperhomocysteinemia （HHcy） were showed the depression and the atherosclerosis to a visible extent.