喜马拉雅旱獭肝穿刺方法的建立

Establishment of Liver Puncture Method in Marmota himalayan

目的建立喜马拉雅旱獭活体肝穿刺方法。方法动物经盐酸氯胺酮（45mg／kg）肌肉注射麻醉后，活检穿刺枪于动物右侧上腋中线第3、4肋间，距离胸骨剑突下方1．5cm，肝实音区进针，快速采集肝组织。对20只旱獭施行了肝穿刺，间隔15d穿刺1次，共80次。结果80次组织穿刺取得组织72份，穿刺成功率90％。HE染色证实72例穿刺组织均为肝组织，其中70例穿刺组织在镜下可见至少3个门管区。因肝穿刺导致的旱獭死亡2例，死亡率10％。结论该法操作简便，定位准确，可间隔重复采集肝组织，适用于旱獭肝组织病理学研究。

Objective To establish the method of liver puncture in marmota himalayan. Method The animal was anesthetized by ketamine hydrochloride （45mg/kg） intramuscular injection. Liver puncture point was on the right side of the abdomen in the third and fourth ribs of animals used by biopsy puncture gun, below the sternum xiphoid 1.5 cm, with parallel the midpoint of axillary. Twenty marmota himalayan were punctured with the interval 15days, by total of eighty times from 2009 to 2010. Result Seventy-two liver tissue samples were collected and the success rate was 90%. HE staining confirmed that 70 cases of biopsy tissue had at least three portal areas in the microscope. There were two marmots died caused by liver biopsy and the death rate was 10%. Conclusion Successfully established the liver puncture method of marmota himalayan animal models of hepatitis B, the method was simple, accurate, repeated sampling.