负压封闭引流技术对兔外周血炎症因子的影响

Effect of vacuum sealing drainage on inflammatory factors in peripheral blood in rabbit

目的观察负压封闭引流技术(VSD)对兔创面模型外周血白细胞(WBC)计数、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)含量的影响。方法建立兔创面模型并实施VSD手术(负压组),于7天内各时间点(6、12小时、1、3、5、7天)抽取静脉血,检测WBC计数、CRP含量、TNF-α和IL-6含量。并设立常规换药实验兔(常规组)进行对照。结果建模前两组外周血WBC计数、CRP、TNF-α和IL-6含量均无显著性差异(P>0.05)。建模后两组指标含量均不同程度增高,自6小时时间点起负压组的外周血WBC计数、CRP和IL-6含量较常规组显著性降低(P<0.05);自1天时间点起负压组的外周静脉血TNF-α含量较常规组显著性降低(P<0.01)。结论 VSD技术可能通过引流创面坏死组织和渗出液,下调炎症反应,早期降低外周血WBC计数、CRP、TNF-α和IL-6含量,避免系统性炎症反应的发生。

Objective This study aimed to investigate the effect of vacuum sealing drainage（VSD）on peripheral inflammation indicators or factors including white blood cell（WBC）,C reactive protein（CRP）,tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） of wound rabbit model.Methods Twelve wound rabbit models were performed the VSD（VSD group）,then venous blood was drawn at different time points within 7 days（6th h,12th h,1st d,3rd d,5th d,7th d）.The number of peripheral WBC was counted by automatic blood cell analyzer,content of CRP was measured by BNⅡ special protein analyzer,content of peripheral TNF-α and IL-6 were measured by ELISA.And the VSD group was compared with the conventional group.Results The number of WBC,content of CRP and IL-6 of VSD group were significantly lower than that of conventional group after 6 hours（P0.05）.The content of TNF-α of VSD group was significantly lower than that of conventional group after 24 hours（P0.01）.Conclusion The number of WBC,content of CRP,TNF-α and IL-6 can decrease by proper suction of inflammatory fluids,decrease the inflammatory response,resulting in avoiding the systemic inflammatory response.