摘要以2%可溶性淀粉为唯一碳源的改良查氏液体培养基诱导发酵外生菌根食用菌松口蘑[Tricholoma matsutake(S.Ito et Imai)Sing.];采用3,5-二硝基水杨酸(DNS)比色法检测到松口蘑产生淀粉酶,其活力为0.226U/mL,约为香菇(Lentinula edo-des)淀粉酶活力的7%。结果证明了松口蘑水解利用淀粉的能力,展示了人工驯化松口蘑的潜力。
1Hirato H,Kitamoto Y.Effect of physico-chemical characteristics of the culture substrate and nutritional conditions on mycelial growth in Tricholoma matsutake[J].Mushroom Sci Biotechnol,1995,2:17-22.
2Ohta A.Ability of ectomycorrhizal fungi to utilize starch and related substrates[J].Mycoscience,1997,38:403-408.
3Kusuda M,Nagai M,Hur TC,Ueda M,et al.Purification and some properties of α-amylase from all ectomycorrhizal fungus,Tricholoma matsutake[J].Mycoscience,2003,44:311-317.
4沈萍,范秀荣,李广武.微生物学实验[M].3版.北京:高等教育出版社,2008:214-215.
5Hitoshi Murata,Katsuhiko Babasaki,Akiyoshi Yamada.Highly polymorphic DNA markers to specify strains of the ectomycorrhizal basidiomycete Tricholoma matsutake based on σ marY1 , the long terminal repeat of gypsy-type retroelement marY1[J].Mycorrhiza.2005(3)
6Norihisa Matsushita,Kensuke Kikuchi,Yasumasa Sasaki,Alexis Guerin-Laguette,Frédéric Lapeyrie,Lu-Min Vaario,Marcello Intini,Kazuo Suzuki.Genetic relationship of Tricholoma matsutake and T. nauseosum from the Northern Hemisphere based on analyses of ribosomal DNA spacer regions[J].Mycoscience.2005(2)
7Akiyoshi Yamada,Hisayasu Kobayashi,Hitoshi Murata.Tricholoma matsutake IFO6933 and IFO30604, “matsutake” isolates that have been maintained on slants and widely used in vitro for a quarter to half a century, can form ectomycorrhiza in Pinus densiflora[J].Mycoscience.2003(3)
8Thomas R. Horton.Molecular approaches to ectomycorrhizal diversity studies: variation in ITS at a local scale[J].Plant and Soil (-).2002(1-2)
9W. M. Gill,F. Lapeyrie,T. Gomi,K. Suzuki.Tricholoma matsutake– an assessment of in situ and in vitro infection by observing cleared and stained whole roots[J].Mycorrhiza.1999(4)
10R. Kalendar,T. Grob,M. Regina,A. Suoniemi,A. Schulman.IRAP and REMAP: two new retrotransposon-based DNA fingerprinting techniques[J].TAG Theoretical and Applied Genetics.1999(5)
