红砂愈伤组织适应盐胁迫的渗透调节机制研究

Study on Mechanisms of Osmo regulation of Reaumuria soongorica Callus in Adapting to Salt Stress

测定在不同盐胁迫下红砂愈伤组织中几种渗透调节物质的累积量,研究盐胁迫下红砂的渗透调节机制。研究结果表明,在盐胁迫下,红砂愈伤组织的相对生长率随着盐浓度的增加先升高后降低。红砂愈伤组织具有很强的耐盐能力,低于100 mM NaCl处理可促进红砂的生长,大于100 mM NaCl处理,则会抑制红砂生长。红砂愈伤组织中脯氨酸、甘氨酸甜菜碱、类黄酮和海藻糖在盐胁迫下大量累积,是红砂渗透调节能力改善的积极反应,也是红砂耐盐性强的体现和重要原因。

Reaumuria soongorica（Pall.） Maxim.,a perennial semi-shrub,is widely found in semi-arid areas in northwestern China.It is a resurrection plant that can tolerate prolonged protoplast desiccation conditions and revive upon rehydration.The present study aimed to investigate the salt tolerance mechanisms of calluses induced from R.soongorica.The growth and organic osmolytes under salt stress were examined.Results were summarized as follows.The relative growth rate of R.soongorica callus reached the maximum in 14 days presence of 100 mM NaCl and it was inhibited with further increasing of NaCl concentrations.The callus had higher relative growth rate in low salt concentrations than in high NaCl concentrations or no NaCl,which indicated that R.soongorica callus need salt in the progress of R.soongorica growing.Examination of the osmotic substances under salt stress showed accumulation of proline,glycine betaine and trehalose with increasing of NaCl concentrations,and the strong osmotic adjustment ability was a important factor in the salt resistant mechanism.The results indicated that the calluses of R.soongorica retained similar to other halophytes in response to salt stress.