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HPLC-远紫外吸收光谱法测定盐酸美金刚胶囊的溶出曲线

HPLC Far-UV absorption spectra determination of dissolution curve of Memantine hydrochloride capsules
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摘要 目的建立盐酸美金刚胶囊的溶出曲线测定方法。方法选用Lichrospher二醇基色谱柱(25cm×4mm,5μm),以0.34%磷酸氢二钾溶液-乙腈(60∶40)为流动相,流速0.8ml/min,检测波长为194nm。结果盐酸美金刚线性范围10.0~60.1μg/ml(r=0.9991),重复性试验RSD为1.9%,平均回收率99.3%。结论本方法测定简便,可用于该制剂的溶出曲线测定。 Objective To establish an HPLC Far-UV absorption spectra method for the determination of the dissolution curve of Memantine hydrochloride capsules.Methods Performed by a Lichrospher Diol column(25cm×4mm,5μm),and the mobile phase is consisted of 0.34% Potassium phosphate dibasic solution-acetonitrile(60:40) at a flow rate of 0.8ml/min.The detection wave was set at 194 nm.Results An excellent separation is achieved for Memantine hydrochloride.The assay is validated in the concentration range of 10.0~60.1μg/ml(r=0.9991) and the average recovery was 99.3%.Conclusions The method development is successfully used on the dissolution curve of Memantine hydrochloride capsules.
作者 郑子栋
出处 《医药论坛杂志》 2011年第16期24-26,共3页 Journal of Medical Forum
关键词 盐酸美金刚胶囊 溶出曲线 高效液相色谱法 远紫外吸收光谱法 Memantine hydrochloride capsules Dissolution curve HPLC Far - UV
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参考文献5

  • 1Koeberle M,Hughes P,Wilson C,et al. Development of a liquid chromatography - mass spectrometric method for measuring the binding of memantine to different melanins[J]. J Chromatogr B: Analyt Technol Biomed Life Sci,2003, 787(2) :313-322.
  • 2Leis HJ,Fauler G, Windischhofer W. Quantitative analysis of Memantine In human plasma by gas chromatography - negative ion chemical ionization/mass spectrometry[J]. J Mass Spectrom, 2002,37 (5): 477-480.
  • 3周杏琴,钦晓锋,邹美芬,朱钧清,曹国宪.美金刚胺片的HPLC-ELSD测定[J].中国医药工业杂志,2004,35(11):675-676. 被引量:9
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二级参考文献3

  • 1Koeberle M, Hughes P, Wilson C, et al. Development of a liquid chromatography-mass spectrometric method for measuring the binding of memantine to different melanins [J]. JChromatogr B: Analyt Technol Biomed Life Sci, 2003, 787(2): 313-322.
  • 2Leis HJ, Fauler G, Windischhofer W. Quantitative analysis of memantine in human plasma by gas chromatography-negative ion chemical ionization/mass spectrometry [J]. J Mass Spectrom, 2002, 37 (5): 477-480.
  • 3Duh TH, Wu HL, Kou HS, et al. (2-Naphthoxy) acetyl chloride,a simple fluorescent reagent [J]. J Chromatogr A, 2003, 987(1-2): 205-209.

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