摘要
本试验选择高表达密码子重新设计合成猪β干扰素成熟肽核苷酸,通过设计特异的引物,在毕赤酵母表达系统中表达PoIFN-β,获得分泌表达的高活性重组PoIFN-β,并采用非洲绿猴肾细胞(Vero)-水泡性口炎病毒(VSV)系统以细胞病变抑制法测定重组猪β干扰素的效价,为指导重组PoIFN-β的临床使用和研究其生物学功能提供基础。
The mature nucleotide peptide of porcine interferon-β was redesigned and synthesized by high expression codon in the test,designed specific primers,porcine interferon-β was expressed through Pichia pastoris expression system,recombinant PoIFN-β was measured by cytopathic effects with Vero-VSV system,the clinical application and biological function of recombinant PoIFN-β were provided by the research.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第9期125-129,共5页
China Animal Husbandry & Veterinary Medicine
基金
广东温氏食品集团有限公司科研基金资助(C080711)
关键词
猪β干扰素
酵母表达
高活性
porcine interferon-β
expression in Pichia pastoris
high activity
