大连地区奶牛隐性乳房炎主要病原菌的分离鉴定及药敏试验

Isolation,Identification and Drug Sensitivity Test of the Pathogenic Bacteria of Cow Subclinical Mastitis in Dalian Area

为了明确引起大连地区奶牛隐性乳房炎的主要病原菌及其发病情况,对大连市不同地区的256头黑白花泌乳奶牛进行调查研究,对奶牛隐性乳房炎奶样进行病原菌的分离鉴定及药敏试验。结果表明,检出奶牛隐性乳房炎阳性率为65.63%、乳区阳性率为51.95%;病原菌以金黄色葡萄球菌(Staphylococcus aureus)、链球菌(Streptococcus)和大肠杆菌(Escherichia coli)为主,占检出细菌总数的83.33%;体外药敏试验筛选出了对主要病原菌高度敏感的药物,能有效地治疗奶牛隐性乳房炎。

In order to clarify the major pathogenic bacteria and the incidence of cow subclinical mastitis in Dalian area,256 cows of the different lactation time were investigated from different areas of Dalian.The major pathogenic bacteria were isolated and identified from the milk samples of the subclinical mastitis in dairy cows,and the susceptibility to antibiotics for the major pathogenic bacteria was tested.The results showed that the positive rate of subclinical mastitis was 65.63% and the mammary region positive rate of subclinical mastitis was 51.95% in the tested cows;Staphylococcus aureus,Streptococcus and Escherichia coli were the major pathogenic bacteria causing cow subclinical mastitis,and the cases of cow subclinical mastitis caused with this three kinds of pathogenic bacteria were 83.33%;the hypersensitive antibiotics to the major pathogenic bacteria of cow subclinical mastitis were screened by the drug sensitivity test in vitro,these hypersensitive antibiotics could efficiently treat cow subclinical mastitis in Dalian area.