复方中药制剂的抗炎及毒性作用检测

Test on the Anti-inflammatory Action and the Toxic Effect of Chinese Herbal Medicine Prescription

采用小鼠耳廓肿胀法和小鼠肉芽组织增生法研究治疗奶牛隐性乳房炎的复方中药制剂的抗炎效果;采用改进Kar-ber法,以半数致死量(LD50)作为评价急性毒性的指标,研究复方中药制剂的急性毒性作用。结果表明,复方中药制剂的低、中、高剂量组对小鼠耳廓的肿胀度(分别为10.41、9.26、8.82mg)与对照组(生理盐水)的肿胀度(12.52mg)相比差异显著(P<0.05);复方中药制剂组小鼠的棉球致肉芽肿增重分别为11.21、10.71、8.83mg,与对照组增重(12.63mg)相比差异显著(P<0.05)。在急性毒性试验中无小白鼠死亡,LD50>60000mg/kg体重。研究表明复方中药制剂能有效对抗急性和慢性炎症,抗炎效果显著,且该药安全可靠,可作为临床用药。

The methods of mice ear-swelling and granulation tissue hyperplasia of mice induced by tampon were adopted to study the anti-inflammatory action of pure traditional Chinese herbal medicine for dairy cow mastitis,and improved Karbr was adopted to study the toxic effect of the Chinese herbal medicine prescription with LD50 as the evaluate index of the acute toxicity strength.The test results indicated that the Chinese herbal medicine prescription to mice ear swell were 10.41,9.26,8.82 mg,respectively in the three test groups（high dosage,middle dosage and low dosage of the prescription）.There had significant difference between the control group（12.52 mg） and test groups（P0.05）.Mice granulation tissue hyperplasia in the Chinese herbal medicine prescription groups were 11.21,10.71,8.83 mg,respectively.There had significant difference between the control group（12.63 mg） and test groups（P0.05）.In the actute toxicity test,none mouse was dead,so the LD50 of the prescription were more than 60000 mg/kg·bw.The results showed that the anti-inflammation action of the Chinese herbal medicine prescription was significantly effective for both of active and chronic inflammation period.The Chinese herbal medicine prescription belongs to a practical innoxious substance and can be used in the clinical research.