HPLC法测定板蓝根片中尿苷和腺苷的含量

Determination of Guanosine and Andadenosine in Banlangen Tablets by HPLC

目的:建立板蓝根片中尿苷和腺苷的HPLC含量测定方法,为提高该制剂质量标准提供科学依据。方法:色谱柱:Wa-ters Sunfire C18(4.6×250 mm,5μm);流动相为A(甲醇)-B(水),梯度洗脱(0～23 min,A:4%→20%,B:96%→80%);检测波长:260 nm。结果:尿苷和腺苷分别在0.010～0.208μg和0.001～0.021μg范围内与峰面积呈良好线性关系,r均为0.999 9;平均回收率分别为98.94%和99.79%,RSD值分别为1.1%和2.2%。结论:该方法简便易行,具有良好的重复性和稳定性,可用于板蓝根片质量控制。

Objecitve：To develop a method for determining guanosine and andadenosine in Banlangen Tablets by HPLC. To improve the preparation to provide the scientific basis for quality standards. Methods：Analytical column：Waters SunFire TM C 18 （4. 6 × 250mm, 5μm） ;The mobile phase consisted of A （methanol） and B（water） with gradient elution （0 - 23 min, A ：4% →20%, B ：96% →80% ） ; The detection wavelength was at 260 nm. Results： The method had good linear relationship within the range of 0. 010 ~ 0. 208μg and 0. 001 ~ 0. 021 μg for guanosine and andadenosine, respectively, with the correlation coefficients of 0. 999 9, the average recoveries were 98. 94% （RSD = 1.1% ）and 99. 79% （ RSD = 2. 2% ）. Conclusion：This method was simple, and has good repeatability and stability, and can be used for the quality control of Banlangen Tablets.