MDR1基因甲基化与新疆维吾尔族、汉族宫颈癌新辅助化疗疗效的相关性

Methylation of MDR1 gene in promoter region is related with the effect of neoadjuvant chemotherapy on cervical carcinoma in Uigur and Han of Xinjiang

目的:探讨多药耐药基因(MDR1)启动子区的甲基化状态与新疆维吾尔族、汉族宫颈癌新辅助化疗疗效的相关性。方法:采用MassARRY EpiTYPER DNA甲基化分析技术定量分析宫颈鳞癌61例(汉族:39例,维吾尔族:22例)新辅助化疗前后及正常宫颈组织50例(汉族:30例,维吾尔族:20例)中MDR1基因启动子区22个CpG位点的甲基化状态。结果:(1)宫颈癌组的平均甲基化率均低于正常宫颈组,汉族除了CpG-11,CpG-12、13,CpG-23,CpG-30,维吾尔族除了CpG-5,CpG-11,CpG-12、13,CpG-19、20,CpG-22,CpG-23,CpG-25、26、27外,其余CpG单位差异均有统计学意义(P<0.05)。(2)在维吾尔族、汉族化疗前宫颈癌平均甲基化率比较中发现,CpG-5,CpG-7,CpG-8,CpG-19、20,CpG-23,CpG-25、26、27,差异有统计学意义(P<0.05)。(3)宫颈癌新辅助化疗后的平均甲基化率低于化疗前,汉族CpG-7,CpG-8,CpG-12、13,CpG-18,CpG-23及维吾尔族的CpG-7,CpG-8,CpG-12、13,CpG-18,CpG-19、20,CpG-25、26、27,CpG-30差异有统计学意义(P<0.05)。(4)新辅助化疗耐药组的平均甲基化率低于敏感组,汉族CpG-2、3、4及维吾尔族CpG-6,CpG-7,CpG-8,CpG-12、13,CpG-19、20,CpG-23,CpG-30差异有统计学意义(P<0.05)。结论:MDR1基因启动子区部分CpG岛的异常甲基化与宫颈癌的新辅助化疗疗效密切相关,且在维吾尔族、汉族之间存在一定差异。

Objective：To investigate the correlation between methylation of MDR1 gene in promoter region and the effect of neoadjuvant chemotherapy on cervical carcinoma in Uigur and Han of xinjiang.Method：Mass ARRY EpiTYPER DNA methylation analyzing technique was used to detect the 22 CPG sites methylation status of MDR1 gene promoter region which in groups before and after NACT of cervical carcinoma and normal group.Results：（1）The average methylation rate in cervical carcinoma group was lower than that in the normal group,except CpG-11,CpG-12,13,CpG-23,CpG-30 of Han and CpG-5,CpG-11,CpG-12,13,CpG-19,20,CpG-22,CpG-23,CpG-25,26,27 of Uigur（P0.05）.（2）We found the average methylation rate of CpG-5,CpG-7,CpG-8,CpG-19,20,CpG-23,CpG-25,26,27 have significant difference in Uigur and Han in before NACT group（P0.05）.（3）The average methylation rate in after NACT group was lower than that in before NACT group,CpG-7,CpG-8,CpG-12,13,CpG-18,CpG-23 of Han and CpG-7,CpG-8,CpG-12,13,CpG-18,CpG-19,20,CpG-25,26,27,CpG-30 of Uigur significant difference were found（P0.05）.（4）The average methylation rate in drug resistance group was lower than that in sensitive group,CpG-2,3,4 of Han and CpG-6,CpG-7,CpG-8,CpG-12,13 CpG-19,20 CpG-23 CpG-30 of Uigur significant difference was found（P0.05）.Conclusion：There is closely correlation between parts CPG island abnormal methylation of MDR1 gene in promoter region and the effect of neoadjuvant chemotherapy on cervical carcinoma,and some differences were also found in Uigur and Han.