摘要
A群轮状病毒(GAR)是引起人类和多种动物腹泻的主要病原体,病原的分离与鉴定是轮状病毒(RV)流行病学、病原学等研究的基础。本研究采用接种MA104细胞的方法,从内蒙古某猪场患病猪腹泻粪便中分离一株病毒,经3次蚀斑克隆纯化后,采用电镜观察、PCR鉴定和序列测定进行分析,结果表明该分离株为猪轮状病毒(PoRV)。致病性试验表明该分离株能够引起仔猪急性腹泻,RT-PCR扩增其VP4、VP6和VP7的基因节段并进行序列测定,按照A群RV的最新分类方法,确定该分离株的VP6、VP7和VP4基因型分别为I5型、G9型和P[23]型。因此,将该分离株命名为Rotavirus A pig/China/NMTL/2009/Q9P[23]。
Group A rotaviruses are the main pathogens which cause diarrhea in human and animals.An virus was isolated from piglet with diarrhea in Inner Mongolia and purified 3 times in MA104 cell.The virus was identified to be porcine rotavirus by electron microscopic observation,PCR and sequencing analysis,designated A pig/China/NMTL/2009/G9P[23].The pathogenicity experiment showed that the virus caused acute diarrhea in piglet.According to the sequence analysis,the virus was classified as group A rotaviruses,the genotype of VP4/6/7 was belonged to P[23],I5,and G9,respectively.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2011年第9期681-684,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
哈尔滨市攻关项目(2009AA6AN029)
关键词
猪轮状病毒
分离
鉴定
porcine rotavirus
isolation
identification
