白藜芦醇对呼吸道合胞病毒感染小鼠肺部病毒滴度及其气道炎症的影响

Effect of resveratrol on pulmonary vurus titer and airway inflammation in mice infected with respiratory syncytial virus

目的研究白藜芦醇(resveratrol,RES)对呼吸道合胞病毒(respiratory syncytial virus,RSV)感染小鼠肺部病毒滴度及其所致气道炎症的影响。方法 6～8周龄雌性BALB/c小鼠,环磷酰胺100 mg/kg腹腔注射后,随机分为对照组、RSV感染组、白藜芦醇腹腔注射组、白藜芦醇雾化组,每组8只。环磷酰胺处理5 d后,给予RSV滴鼻建立RSV感染模型,并在RSV感染1 h后给予白藜芦醇30 mg/(kg.d)腹腔注射和雾化吸入。5 d后检测小鼠清醒状态下气道阻力,处死小鼠研究肺部RSV病毒滴度、肺泡灌洗液(BALF)中细胞计数、肺部病理组织炎症及评分。结果白藜芦醇不同方式给药,干预组肺部RSV病毒滴度均显著低于RSV感染组(P<0.01),BALF中细胞总数白藜芦醇干预组明显低于RSV感染组(P<0.05),而白藜芦醇干预组与对照组相比差异无显著性;肺组织炎症病理评分:白藜芦醇干预组明显低于RSV感染组(P<0.01),白藜芦醇干预组清醒状态小鼠气道高反应(吸入乙酰甲胆碱浓度12.5～50.0 mg/ml时)较单纯RSV感染组明显减轻(P<0.05)。结论白藜芦醇不同方式给药均能够减轻RSV诱发的肺部炎症及气道高反应,其机制可能是通过抑制RSV感染后肺部病毒滴度,减少RSV在体内的复制。

Objective To study the effect of resveratrol（RES） on pulmonary virus titer and airway inflammation in mice infected with respiratory syncytial virus（RSV）.Methods Twenty-four female BALB/c mice at the age of 6-8 weeks,treated with intra-abdominal cyclophosphamide（CYP） at the dose of 100 mg/kg,were randomly divided into control group,RSV infection group,and RES treatment group（8 in each group）.Five days after CYP treatment,a RES infection model was induced by intranasal drip of RES.One hour after RES infection,the mice received intraperitoneal injection of RES or inhaled it at the dose of 30mg/（kg·d）.Five days later,airway hyper-response（AHR） in lucid mice was detected.The mice were then killed to detect the pulmonary RSV titers in the lung,the number of cells in bronchoalevolar lavage fluid（BALF）,inflammation in lung tissue and its score.Results The pulmonary RSV titer,the number of cells in BALF,the score of inflammation in lung tissue,and the airway AHR were significantly lower in RES treatment group than in RSV infection group（P0.01）.However,no significant difference was observed between RES treatment group and control group.Conclusion Differenly administrated RES can relieve RSV-induced inflammation and AHR by reducing pulmonary titer and inhibiting RSV replication in vivo.