卡介苗对hPBMC的TLR4表达调节及其免疫活化作用的研究

TLR4 expression of human PBMC treated by BCG and its role of immune activation

目的:了解卡介苗对人外周血单个核细胞(hPB-MC)TLR4的表达调节,以及BCG介导免疫细胞活化效应的新机制。方法:研究BCG对hPBMC的TLR4表达的调节,以及对表达TLR4的淋巴细胞的免疫活化效应,以(hPBMC+PBS)作为空白对照组,应用流式细胞仪对TLR4进行检测,ELISA方法测定BCG刺激组与对照组的IFN-γ和TNF-α的表达。结果:经过BCG刺激后,TLR4表达大大增加(P<0.01),且随时间增加而增强,在72 h时,BCG组的TLR4表达率为(44.73±0.0066)%,而对照组的表达率仅为(1.02±0.0024)%。BCG可以促进淋巴细胞增殖,且这种增强作用也存在一定时间依赖性,在BCG与hPBMC共同孵育24 h、48h和72 h后,BCG组IFN-γ和TNF-α的表达量显著高于对照组(PBS组),差异有统计学意义(P<0.05),且这种增强作用存在一定的时间依赖性。结论:BCG对hPBMC的TLR4表达有正调节作用,并加强表达TLR4的hPBMC的免疫活化作用。

AIM： To investigate TLR4 expression of human peripheral blood mononuclear cells（hPBMC） treated with BCG and its role of immune activation.METHODS： hPBMC were treated with BCG in vitro.TLR4 expression were detected by flow cytometry,IFN-γ and TNF-α expression of hPBMC in both BCG stimulated group and the control group were detected by ELISA.RESULTS： The expression of TLR4 in hPBMC treated with BCG was stronger than the control group significantly（P0.01） and increased with the time.In 72 h the TLR4 expression of BCG group was（44.73±0.0066）%,while the control group was（1.02±0.0024）%.BCG can promote hPBMC proliferation,and this enhancement was time-dependent.In 24 h,48 h and 72 h IFN-γ and TNF-α expression of BCG group were significantly higher than the control group（P0.05）,and this enhancement was time-dependent.CONCLUSION： BCG can on enhance TLR4 expression and promote immune activation of hPBMC.