姜黄素对IL-17诱导的人角质形成细胞NO合成以及iNOS表达的影响

Effect of curcumin on IL-17-induced nitric oxide production and expression of iNOS in human keratinocytes

目的:探讨姜黄素对IL-17诱导的人表皮角质形成细胞株(HaCaT细胞)NO合成以及诱导型一氧化氮合酶(iN-OS)的mRNA和蛋白表达的影响。方法:用IL-17刺激体外培养的HaCaT细胞,并分别加入3种浓度的姜黄素共培养24 h。并收集细胞上清液、提取细胞总RNA、总蛋白,分别进行NO含量的测定、荧光定量PCR和W estern b lot实验,明确姜黄素对NO含量以及iNOS表达的影响。结果:IL-17能够有诱导HaCaT细胞NO以及iNOS的表达(P<0.01)。姜黄素有效下调NO合成量以及iNOS的mRNA(P<0.01)及蛋白表达(P<0.01)水平。结论:姜黄素对IL-17诱导的HaCaT细胞NO分泌及iNOS的表达具有明显的抑制作用,从而为其对角质形成细胞相关的皮肤炎症性疾病的治疗提供了理论依据。

AIM： To investigate the effect of curcumin on IL-17-induced NO production,mRNA and protein expression of iNOS in human keratinocyte cell lines（HaCaT cells）.METHODS： HaCaT cells were stimulated with IL-17 and incubated with three doses of curcumin for 24h in vitro.After collections of supernatant,total RNA and protein,NO levels in supernatant were detected and fluorescence quantitative PCR and Western blot were performed to determine the effect of curcumin on NO levels and iNOS.RESULTS： IL-17 increased NO levels,and expression of iNOS in HaCaT cells（P0.01）.Curcumin decreased IL-17 induced NO production and the iNOS expression at mRNA（P0.01） and protein（P0.01） levels significantly.CONCLUSION： Curcumin down-regulates IL-17-induced NO secretions and iNOS expression in HaCaT cells,thus provides a theoretical basis for the treatment of inflammatory diseases of skin related to keratinocytes.