出血性脑损伤患者脑脊液和血清中S100β蛋白的意义

The clinical significance of S100β protein in cerebrospinal fluid and serum from the patients with cerebral hemorrhage

目的:观察脑出血患者CSF和血清中S100β蛋白含量的变化及其临床意义。方法:以25例腹股沟疝和大隐静脉曲张患者作为25例脑出血患者对照组,取CSF和血清;将24只家兔随机分为两组(每组12只),一组实验组,另一组为假手术组。分别于实验性脑出血后6 h、12 h、24 h、48 h、72 h、96 h等各时间点取各组CSF和血清,采用ELISA法测定S100β蛋白的含量。结果:脑出血患者急性期与恢复期脑脊液中S100β蛋白的水平明显高于对照组(P<0.01);也明显高于血清S100β蛋白的水平(P<0.01);不同时间点兔脑出血模型实验组脑脊液中S100β蛋白的水平明显高于假手术组(P<0.01)。结论:脑出血后S100β蛋白在脑脊液中持续时间较长,且含量显著增高,可作为出血性脑损伤的早期诊断、指导治疗、判断预后的检测指标。

AIM： To observe the change and the clinical significance of S100β protein level in cerebrospinal fluid and serum from the patients with cerebral hemorrhage（CH）.METHODS： ELISA was used to detect the expression of S100β protein in CSF and serum from CH patients control with Inguinal Hernia or great saphenous varix patients.Meanwhile,rabbit CH model at 6 h,12 h,24 h,48 h,72 h and 96 h.RESULTS： The levels of CSF S100β protein at acute stage of CH patients increased significantly compared with those at recovery stage of CH patients and control group（P0.01）.The levels of S100β protein in CSF from CH patients increased significantly compared with those in serum（P0.01）.The levels of S100β protein in CSF of rabbit experimental group increased significantly compared with those of sham operation group at different time points（P0.01）.CONCLUSION： The level of S100β protein in CSF from CH patients increases.It may be a biomarker as reflecting degree of pathogenetic and predicting outcome in the CH patients.