人参皂苷Rg1对体外培养C2C12成肌细胞凋亡的影响

Effect of ginsenoside Rgl during serum-deprivation induced apoptosis in C2C12 myoblasts cultured in vitro

目的：研究人参皂苷Rg1对体外无血清诱导培养的C2C12成肌细胞凋亡的影响及其可能机制。方法：采用MTT法、人参皂苷Rg1处理48h后hoechst33258－PI染色，以及RT-PCR方法观察不同浓度人参皂苷Rg1对C2C12成肌细胞凋亡的影响。结果：MTT法结果显示人参皂苷Rg1处理48h后可抑制C2C12成肌细胞凋亡；Hoechst33258－PI染色可见C2C12成肌细胞凋亡率人参皂苷处理前后差异有统计学意义，人参皂苷处理后C2C12成肌细胞凋亡率显著下降；RT-PCR法结果显示人参皂苷Rg1可抑制Caspase-3、Bax和AIFmRNA表达，并能诱导Bcl－2mRNA表达。结论：人参皂苷Rg1对C2C12成肌细胞凋亡具有保护作用。

Objective： To investigate the effect and possible mechanism of ginsenoside Rgl during serum-deprivation induced apoptosis in C2C12 myoblasts cultured in vitro. Methods： The effect of different concentrations of ginsenoside Rgl during the cell apoptosis was assessed by MTT assay, Hoechst 33258-PI double staining and RT-PCR analysis. Results： After 48 h treatment, various doses of ginsenoside Rgl increased cell viability in serum-deprived C2C12 myoblasts using MTT assay. Hoechst 33258-PI double staining showed that the rate of apoptosis cells significantly decreased after being treated by ginsenoside Rgl. RT-PCR showed that ginsenoside Rgl caused the downregulation of pro-apoptotic caspase-3, Bax and AIF genes, while caused the up-regulation of anti-apoptotic Bcl-2 gene. Conclusion; Ginsenoside Rgl can protect the serum-deprived apoptosis in C2C12 myoblasts.