电针对单纯性肥胖大鼠无排卵机制的影响

Mechanism underlying the electric acupuncture improves the anovulation in simple obesity rats

目的：探讨电针对食源性肥胖大鼠排卵机制的影响。方法：将刚断乳雌性SD大鼠分为2组，正常组喂以普通饲料，高能饲料组给予高能饲料，筛选出肥胖不孕大鼠。检测血清雌二醇（E2）、睾酮（T）、瘦素（1eptin）、卵泡刺激素（FSH）、黄体生成素（LH）的水平；观察神经肽Y（NPY）及促性腺激素释放激素（G根H）在下丘脑弓状核的表达情况。结果：电针组较对照组大鼠血清E2、T、leptin水平降低，FSH、LH水平升高；下丘脑弓状核NPY表达减弱、GnRH表达增强。结论：针刺能够调节单纯食源性肥胖大鼠的神经内分泌代谢，从而降低由单纯性肥胖所致的下丘脑一垂体一卵巢轴功能失调性无排卵而引起的不孕比例。

Objective： To explore the effects of electric acupuncture on the ovulating mechanism in obese rats induced by high fat diet. Methods：The obese infertile rats were duplicated by fed high-energy diet to ablactated female SD rats, and then, were randomly untreated used as control or treated with electric acupuncture. The expression of neuropeptide Y （NRY） and gonadotropin-releasing hormone （GnRH） in the arcuate nucleus of hypothalamus, and levels of estradiol （E2）, testosterone （T）, leptin, follicle stimulating hormone （FSH） and luleinizing hormone （LH） in serum were detected. The parameters above-mentioned were analyzed among the normal rats, which were fed with normal diet, control rats and electric acupuncture treated rats. Results： Compared with control rats, electric acupuncture caused significant decrease in levels of E2, T and leptin, and increase in levels of FSH and LH in serum. In the arcuate nucleus of hypothalamus, the expression of NPY was down-regulated, while the expression of GnRH was up-regulated due to the application of electric acupuncture. Conclusion： Electric acupuncture can depress the proportion of anovulatory infertility induced by obesity through modulating the neuroendocrine metabolism and reducing dysfunction of the hypothalamus-pituitary-ovarian axis.