伊文思蓝检测急性高眼压后大鼠血-视网膜屏障的变化

Changes of blood-retinal barrier of rat following acute high intraocular pressure using Evans blue

目的：检测急性高眼压后大鼠血-视网膜屏障（BRB）的改变。方法：大鼠随机分为正常对照组、实验对照组和实验组（急性高眼压模型）。动物分别存活3h、12h、1d、3d、7d，处死前注射伊文思蓝（EB），共聚焦显微镜检测视网膜铺片和切片中EB的分布；用分光光度计定量视网膜EB的含量。结果：在急性高眼压后视网膜中可见，EB红色荧光斑点主要分布在节细胞层，3h、12h和1d还见于内核层。EB红色荧光斑点在视网膜中央部3h时开始增多，12h达高峰，然后逐渐减少；在视网膜周围部3h时最多，然后逐渐减少。进一步定量检测EB渗漏量呈相同的时空改变。结论：大鼠BRB在急性高眼压后的早期损伤更明显，晚期逐渐恢复；视网膜周围部先于中央部出现BRB严重破坏；BRB损伤主要分布于节细胞层。

Objective： To detect the changes of blood-retinal barrier （BRB） following acute high intraocular pressure （AHIOP） in a rat model. Methods： The rats were randomly divided into normal groups, experimental control groups and experimental groups. The rats of the experimental groups were made into AHIOP model, and then survived for 3 h, 12 h, 1 d, 3 d and 7 d. 3% Evans blue （EB） was injected into the saphenous vein of rats before they were sacrificed. The distribution of EB in the retinal wholemount and retinal slice was observed by using confoeal microscopy and the EB content of retina was measured by using spectrophotometer. Results： EB red fluorescence spots were mainly distributed in the ganglion cell layer around capillaries following AHIOP. In 3 h, 12 h and 1 d, it was also found in the inner nuclear layer surrounding the capillaries. The EB red fluorescence spots of the central retina began to increase in 3 h following AHIOP, reached its peak in 12 h, and then gradually decreased. Most of them in the peripheral retina were found in 3 h after AHIOP, and then gradually decreased. Further quantitative analysis showed the same spatial and temporal changes. Conclusion： BRB damage of rats is more evident in its early stage after AHIOP, and restores gradually in its late stage. The serious breakdown of BRB in the peripheral retina precedes the central retina. BRB damage is mainly distributed in the ganglion cell layer.