不同品系小鼠在三种常见抑郁检测方法中的行为学表现

Different Behavioral Activities of Different Mouse Strains Detected by Three Common Tests for Depression

目的探讨4种不同品系小鼠在3种实验(空场实验、悬尾实验及强迫游泳实验)中的行为学差异,为抗抑郁新药研究中的实验动物选择提供参考。方法利用空场实验检测C57BL/6、BALB/c、ICR、和昆明小鼠的自主活动能力和对新奇环境的探索能力;利用悬尾实验和强迫游泳实验检测它们在应激刺激下的行为绝望状态。结果在空场实验中,BALB/c、ICR和昆明小鼠的运动总路程、运动速度和运动时间明显高于C57BL/6小鼠(P<0.05),ICR和昆明小鼠的直立次数也明显高于C57BL/6小鼠(P<0.05);悬尾实验C57BL/6小鼠的不动时间显著长于其他3种品系小鼠(P<0.05),但是4种品系小鼠在强迫游泳实验中的不动时间差异无显著性。结论 C57BL/6小鼠自发活动量低,对新奇环境的探索能力差,并且在急性应激刺激下容易造成行为绝望,因此C57BL/6小鼠可能适合作为急性应激抑郁模型动物。

Objective To investigate different behavioral activities among four species of mice in three trials（open field test,tail suspension test and forced swimming test）,and provide a reference in the research on new antidepressant.Methods Different locomotion activity and exploration in a novel environment of C57BL /6,BALB/c,ICR,and KM mice were investigated by open field with an automated video detecting system,and tail suspension test and forced swimming test were performed to determine their state of ＂behavioral despair＂.Results In the open field test,the total duration of movement,speed and movement distance of BALB/c,ICR and KM mice were significantly higher than that of C57BL/6 mice（P0.05）,and their number of rearing also was significantly more than that of C57BL/6 mice（P0.05）;the immobility time of C57BL/6 mice was significantly longer than that of the other three species of mice measured by tail suspension test（P0.05）,but the fixed time of the four species of mice in the forced swimming test were not significantly different.Conclusions The results of this study demonstrate that among the four mice strains tested,C57BL/6 strain displays less spontaneous activity and less exploration in a novel environment.They are likely to cause behavioral despair under acute stress stimulation.Therefore,the C57BL/6 mice may be probably the strain of choice as an acute stress animal model for studies of depression.