同种异体大鼠骨髓间充质干细胞移植对放射性空肠损伤的修复作用

Repairing Effect of Allogeneic Rat Bone Marrow Mesenchymal Stem Cells on Jejunal Radiation Damage

目的观察同种异体骨髓间充质干细胞(MSCs)移植对放射性空肠的修复作用。方法全骨髓贴壁体外培养大鼠MSCs并进行DAPI标记。对大鼠行5 Gy X线全腹部照射,每隔72 h照射1次,共5次,制备放射性空肠损伤动物模型。取40只大鼠,随机分成正常对照组、模型及MSCs治疗组及DAP标记组。激光共聚焦显微镜下观察DAPI标记的MSCs在空肠组织中富集情况。病理学观察MSCs对放射性空肠损伤的修复作用。结果正常大鼠空肠黏膜结构清楚,隐窝深遂,腺体丰富,模型组7 d黏膜上皮细胞坏死脱落,隐窝几乎完全破坏,治疗组7 d黏膜坏死组织较少,黏膜增厚,30 d治疗组核分裂相增加,较模型组增加明显。结论成功建立放射性空肠损伤动物模型,MSCs可以促进空肠再生与修复。

Objective To observe the repairing effects of allogeneic bone marrow mesenchymal stem cells（MSCs） on jejunal radiation damage in rats.Methods Rat MSCs were isolated,purified and cultured in vitro.The model of ileal radiation damage was made.Sixty rats were randomly divided into normal control group,model group,and MSCs group（n=12）.The enrichment of MSCs in the jejunum were observed under laser scanning confocal microscope.Pathological changes of the jejunum were examined.Results The structure of normal rat jejunal mucosa was clear,with deep crypts and glands.In the model group at 7 days after irradiation,the epithelial cells were necrotic and the crypts were almost completely destroyed.But mucosal necrosis was less severe,the mucosa got thickening,and mitotic activity was significantly increased in the treatment group,compared with that of model group at 30 days after irradiation.Conclusions The model of jejunal radiation damage is successfully established.MSCs can promote the regeneration and repairment of the jejunal radiation damages.