内蒙古汉族儿童白血病与HLA—DRB1多态性的关联分析

Study on the Association between Leukemia in Children of Han People in Inner Mongolia and Polymorphism of HLA-DRBI Alleles

目的通过对内蒙古地区汉族白血病患儿HLA-DRB1基因多态性的分析,以期寻找白血病的易感基因,有助于进一步了解白血病的相关发病机制。方法采用PCR-SSP(序列特异引物聚合酶链反应)方法,对病例组及健康对照组进行HLA-DRB1基因的分型。采用四格表卡方检验,经SPSS13.0统计软件进行统计学分析。结果病例组HLA-DRB1*09的基因频率与对照组的基因频率差异有统计学意义(P<0.05);病例组中HLA-DRB1*14的基因频率与对照组的基因频率差异具有统计学意义(P<0.05);病例组中HLA-DRB1*07的基因频率与对照组的基因频率差异具有统计学意义(P<0.05)。结论 HLA-DRB1*09和HLA-DRB1*14可能是内蒙古汉族儿童白血病的遗传易感基因型,HLA-DRB1*07可能是内蒙古汉族儿童白血病的遗传拮抗基因型。

Objective we analyse HLA-DRB1 allele polymorphism of leukemia in children of Han people residing in Inner Mongolia in order to determine the susceptible genetic factors of leukemia,which would be useful to study at the relative mechanism of leukemia.Methods The method of DNA amplification with polymerase chain reaction-sequence-specific primers（PCR-SSP） was used to determine alleles of HLA-DRB1 in experimental group and the control group.Utilizingχ~2 test to analyse the collected data in SPSS 13.0 software in the statistic analysis.Results The gene frequency of HLA- DRB1＊09 in experimental group and in the control group are statistical significance.P0.05.The gene frequency of HLA- DRB1＊14 in experimental group and in the control group are significant difference.P0.05.The gene frequency of HLA- DRB1＊07 in experimental group and in the control group are statistical significance（P0.05）.Conclusions There maybe the genetic susceptibility effect of HLA-DRB1＊09 allele and HLA- DRB1＊14 allele in Inner Mongolia Han population on childhood leukemia.While,there maybe the genetic resistance effect of HLA- DRB 1＊07 allele.