摘要
目的:探讨法舒地尔(Fasudil)对敌敌畏中毒大鼠急性肺损伤时血浆肿瘤坏死因子α(TNF-α)和白细胞介素-6(IL-6)水平的影响。方法:Sprague Dawley大鼠30只,随机分成对照组、敌敌畏(dichlorvos,DDVP)中毒组、法舒地尔干预组3组,每组10只,均予以气管切开接呼吸机辅助通气。6h后留取血样用酶联免疫吸附分析(ELISA)法测血浆TNF-α、IL-6水平。取左肺下叶组织,HE染色观察病理学改变。结果:与对照组比,中毒组和干预组大鼠血浆TNF-α、IL-6水平均显著升高(P<0.01),肺组织HE染色有炎症细胞浸润;与中毒组比,干预组TNF-α、IL-6升高程度显著降低(P<0.05),肺损伤程度较轻。结论:法舒地尔能降低敌敌畏中毒大鼠血浆TNF-α、IL-6水平,能显著减轻肺损伤的病理损害,对大鼠敌敌畏中毒性急性肺损伤有明显的保护作用。
Objective：To investigate the effect of fasudil on acute lung injury in rats with Dichlorvos poisoning.Methods： 30 male Spragne-Dawley（SD） rats were randomized into the control group,Dichlorvos poisoning group and Fasudil pretreatment group（n=10each）.Three groups were all supported with breathing machine connected with the tube from incision of trachea.tumor necrotic-α（TNF-α）,and interleukin-6（IL-6） in blood serum were measured six hours later.Histomorphology of lung tissue was observered though HE stain.Results： Compared with the control group,the level of TNF-α and IL-6 of the other two groups were higher（P0.01）.Compared with the poisoning group,the level of TNF-α and IL-6 were significant decreased in the pretreatment group（P0.05）.The histological changes of lung injury in the pretreatment group were weakened.Conclusions： It showed that Fasudil could inhibit the level of TNF-α and IL-6 in serum of rats with acute Dichlorvos poisoning.It could also decrease the histomorphology of lung injury.The protective effection on acute lung injury in rats with Dichlorvos poisoning was obviously.
出处
《中国临床医学》
2011年第4期425-426,共2页
Chinese Journal of Clinical Medicine
基金
国家科技支撑计划专题基金(编号:2006BAI06B01-)
