苦参碱诱导HL-60细胞株凋亡作用的实验研究

Apoptosis of cell line HL-60 induced by matrine

目的研究苦参碱（MAT）诱导HL-60细胞株凋亡的作用及其机制。方法采用CCK-8法检测不同浓度MAT对HL-60细胞的增殖抑制作用；流式细胞术检测细胞凋亡以及线粒体跨膜电位的变化；分光光度法检测Caspase-9活性。结果0．25～2．0mg／mlMAT对HL．60细胞有生长抑制作用，与对照组相比差异有统计学意义（F=67．83，P〈0．05）；MAT处理48h后，细胞凋亡率明显增高，并呈剂量依赖性（t值分别为4．685、6．300、9．641、6．786，均P〈0．05）；1．0mg／mlMAT处理后48h内，细胞线粒体跨膜电位逐渐降低（F=54．83，P〈0．05），Caspase-9活性逐渐升高，呈时间依赖性（F=72．31，P〈0．05）。结论MAT可能通过降低细胞线粒体跨膜电位、激活Caspase-9而诱导HL-60细胞凋亡。

Objective To investigate the apoptosis of cell line HL-60 induced by matrine and its possible mechanism. Methods CCK-8 assay was used to observe the proliferation inhibitation of HL-60 cells treated with matrine. FCM was applied to detect apoptotic cells and mitochondrial transmembrane potential. Spectrophotometry was used to detect the activity of Caspase-9. Results Matrine （0.25-2.0 mg/ml） could significantly inhibit proliferation of HL-60 cells （F=67.83, P 〈0.05）. After 48 hours, the apoptosis rate increased obviously in dose dependence （t = 4.685, 6.300. 9.641, 6.786, P 〈0.05）. Within 48 hours, the mitoehondrial transmembrance potential of HL-60 cells treated with matrine （1.0 mg/ml） gradually decreased （F = 54.83, P 〈0.05）, and the activation of Caspase-9 gradually decreased in time dependence （F = 72.31, P 〈0.05）. Conclusion Matrine can induce apoptosis of HL-60 cells by reducing mitochondrial transmembrance potential and activating Caspase-9.