大孔树脂纯化黑粒小麦麸皮色素及其初步鉴定

Identification and Purification of Pigments in Black Wheat Bran by Macroporous Resin

研究了AB-8型大孔树脂对黑粒小麦麸皮色素的吸附与解吸条件,并利用高效液相色谱法对纯化后的色素成分进行了初步的分析。结果表明:黑粒小麦麸皮色素在AB-8型大孔树脂上的吸附平衡时间为26 h,解吸平衡时间为7h,pH对大孔树脂的吸附率影响不大,pH值为1.0的70%的乙醇溶液可以很好的洗脱色素。在该条件下,测得AB-8型大孔树脂最大吸附率为83.00%,最大解吸率为85.53%。纯化后的色素有4个主要成分,为花色苷类化合物。

Studied adsorption and desorption conditions of AB-8 type macroporous resin to black wheat bran pigment, and HPLC were applied to identify the purified pigments. It was found that the absorption and desorption equilibrium time of black wheat bran in AB-8 type macroporous resin was 26 h and 7h. The affect of pH to macroporous resin adsorption rate is not significant. 70% ethanol solution （pH = 1.0） was used to desorption pigment. In this condition, the AB-8 macroporous resin maximum adsorption rate is 83.00% and the maximum desorption rate is 85.53%. Four main anthocyanins component was found in purified pigments.