压力作用时间对人牙周膜成纤维细胞合成TGF-β1的影响

Time-dependent effects of mechanical stress on the secretion of transforming growth factor-β1 from human periodontal ligament fibroblasts

目的:探讨压力作用不同时间对人牙周膜成纤维细胞表达TGF-β1的影响。方法:本实验采用细胞加载装置,对人牙周膜成纤维细胞施加1.0MPa的压力,分别持续10min、30min、50min,通过酶联免疫吸附实验,分别检测细胞受力后6h、12h、18h、24h时TGF-β1的含量。结果:加载10min组在细胞受力后的各个时段TGF-β1含量与对照组相比无显著性差异(P>0.05);加载30min组(0.51±0.06)和加载50min组(0.46±0.06)TGF-β1含量在细胞受力后的第12h同对照组(0.78±0.08)相比明显降低(P<0.05)。结论:在一定加载时间内,人牙周膜成纤维细胞合成TGF-β1量随着压力作用时间的延长而有所降低。

Objective： To investigate the relationship of transforming growth factor-β1（TGF-β1） secreted by human periodontal ligament fibroblasts（HPLF） to duration times of pressure.Methods： We treated cells for 10min,30min and 50min respectively with 1.0MPa pressure by a cellular mechanical research system.TGF-β1 in the supernant was measured at 6h,12h,18h and 24h after stimulation by enzyme link immunosorbent assay（ELISA）.Results： The results showed that cells treated for 10min had no difference in TGF-β1 content compared with control group at different duration times.TGF-β1 of 30min（0.51±0.06） and 50min（0.46±0.06） groups was lower than that of control group（0.78±0.08） at 12h after stimulation respectively.Conclusion： In certain range of duration times of pressure,mechanical pressure can suppress TGF-β1 synthesis by HPLF in a duration-time dependent manner.