mfn2基因在猫爪草皂苷、红三叶异黄酮治疗乳腺癌中的作用机制研究

Mechanism Study of mfn2 Gene in Radix Ranuncoli Ternati Saponins and Red Clover Isoflavone-treated Breast Cancer

目的:研究mfn2基因在猫爪草皂苷、红三叶异黄酮抑制人乳腺癌MCF-7细胞增殖及诱导其凋亡中的作用。方法:采用不同浓度的猫爪草皂苷和红三叶异黄酮分别处理MCF-7细胞24,48及72h,进行体外细胞培养,用实时荧光定量PCR法检测mfn2基因的相对表达情况。结果:高浓度的猫爪草皂苷(100 mg·L^(-1))作用MCF-7细胞48h后,明显促进mfn2基因的表达,并且随时间延长更加明显;红三叶异黄酮在作用MCF-7细胞48,72 h后,显著抑制mfn2基因的表达。结论:高浓度的猫爪草皂苷可能是通过促进mfn2基因的表达,来抑制MCF-7细胞增殖及诱导其凋亡,红三叶异黄酮的作用机制有待于进一步研究。

Objective： To study the mechanism of infn2 gene in radix ranuncoli ternati （RRTS） and red clover isoflavone-treated breast cancer. Method： MCF-7 cells were cuhrued in vitro, then separately treated by different concentration of RRTS （25, 50 and 100 mg· L^- 1 ） and red clover isoflavone （ 50,100 and 200 μg· ml^- 1 ） for 24, 48 and 72 h. The total RNA of each sample was extracted by Tfizol method. The relative expression of mfn2 gene was measured by real-time PCR. Result： There was no significant difference in the expression of mfn2 gene between the control group and low concentration group （25 and 50 mg· L^- 1 ） of RRTS, however the high concentration （100 mg· L^- 1） of RRTS could obviously promote the expression of mfn2 gene in MCF-7 ceils treated for 48 h, and the expression was increased with the treating time. On the contrary, red clover isoflavone inhibited the expression after treating MCF-7 cells for 48 h and 72 h （P 〈0. 01 ）. Conclusion： High concentration of RRTS （ 100mg· L^- 1 ） can inhibit the proliferation and promote the apoptosis of MCF-7 cells through affecting mfn2 gene while the mechanism of red clover isoflavone needs further study.