HPLC测定跌打止痛贴膏中三七皂苷R_1和人参皂苷Rg_1含量

Determination of Notoginsenoside R_1 and Ginsenoside Rg_1 in Dieda Zhitong Plasters by HPLC

目的:建立HPLC法同时测定跌打止痛贴膏中三七皂苷R_1和人参皂苷Rg_1含量的方法。方法:色谱柱为Shim-packCLC-ODS C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.05%磷酸溶液,梯度洗脱;检测波长203 nm,柱温35℃,流速1.0ml·min^(-1)。结果:三七皂苷R_1和人参皂苷Rg_1线性范围分别为0.035 8～0.178 8 mg·ml^(-1)(r=0.999 7),0.225 5～1.128 0mg·ml^(-1)(r=0.999 6);平均回收率分别为96.8%(RSD=2.1%)和97.3%(RSD=2.2%)。结论:本方法简便可靠,重复性好,可用于测定跌打止痛贴膏中三七皂苷R_1和人参皂苷Rg_1的含量。

Objective ： To develop an HPLC method for the determination of notoginsenoside R1 and ginsenoside Rg1 in Dieda Zhitong plasters. Method： The chromatographic separation was performed on a Shim-pack CLC-ODS C18 column （250 mm × 4. 6 mm,5 μm） with a linear gradient elution of aeetonitrile-0. 05% phosphoric acid. UV detection wavelength was set at 203 nm ,the flow rate was 1.0 ml. min ^-1 and column temperature was set at 35 ℃. Result： Notoginsenoside R1 and ginsenoside Rg1 had a good linearity within the range of 0. 035 8-0. 178 8 mg. ml^-1 （ r = 0. 999 7 ） and 0. 225 5-1. 128 0 mg. ml^-1 （ r = 0. 999 6 ） with the average recovery of 96. 8 % （RSD = 2. 1% ） and 97.3 % （RSD = 2. 2% ）, respectively. Conclusion： The method is sensitive, accurate, rapid and suitable for the quality control of Dieda Zhitong plasters.