反相高效液相色谱法检测人血浆帕珠沙星浓度

Determination of Pazufloxacin in Human Plasma by RP-HPLC

目的:建立人血浆帕珠沙星检测的高效液相色谱方法。方法:血浆经高氯酸沉淀,以Agilent TC-C_(18)为色谱柱;流动相为乙腈-0.1%三氟乙酸-水体系,流速为0.8 ml·min^(-1);检测波长314 nm。结果:帕珠沙星浓度在0.10～20.00 mg·L^(-1)范围内线性关系良好(r=0.999 8);高中低三个浓度(0.25,5.00,15.00 mg·L^(-1))的相对回收率分别为(101.47±4.53)%、(99.50±3.63)%和(100.71±3.33)%;日内RSD分别为3.12%、2.85%和2.63%,日间RSD分别为4.18%、3.29%和2.81%。结论:该方法简便、快速、准确可靠,适用于人血浆帕珠沙星浓度的测定及其药物动力学研究。

Objective： To develop an HPLC method for the determination of pazufloxacin in human plasma. Method： The human plasma samples were precipitated by perchloric acid. The analytical column was packed with an Agilent TC-C18 column. The mobile phase was acetonitrile-0. 1% trifluoroacetic acid-water and the flow rate was 0. 8 ml·min^-1. The UV detection wavelength was 314 nm. Result： Excellent linear relationship was obtained within the range of 0. 10 mg·min^-1to 20. 00 mg. L^- 1 （r = 0. 999 8 ）. The relative recovery was （ 101.47 ± 4. 53 ） % , （99. 50 ± 3.63 ） % and （ 100. 71 ± 3.33 ） % , respectively for three concentrations （0. 25,5.00, 15.00 mg.L^-1 ） ,the intra-day RSD was 3.12% ,2. 85% and 2. 63% ,and the inter-day RSD was 4. 18% ,3.29% and 2. 81% ,respectively. Conclusion： The method is simple, rapid and accurate which can be used to determine pazufloxacin in human plasma and study its nharmacokinetics.