香蕉枯萎病拮抗内生菌的分离、筛选及盆栽防效试验

Isolation,screen and potted test of an endophytic bacterial against Fusarium oxysporum

采用组织表面消毒法分离变叶木、益智、阴香、槟榔、椰子和菠萝蜜6种植物的内生菌,获得91株内生菌,其中内生细菌45株,内生真菌38株,内生放线菌8株。以香蕉枯萎病菌(Fusarium oxysporum f.sp.cubense)为靶标菌,对分离的内生菌株进行皿内拮抗活性筛选,发现内生真菌、内生放线菌抑菌效果不理想,内生细菌YXG2-3拮抗效果最好,抑菌带达20mm,其次是BYG2-5、BYJ5-1、YXJ2-2。对4株内生细菌进行盆栽试验,发现菌株YXG2-3防效最好,达到63.8%,与其他处理达极显著水平。

Endophytic bacteria were isolated from Codiaeum variegatum, Alpinia oxyphylla, Cinnamomum burmannii, Areca catechu, Cocos nucifera, Artocarpus heterophyllus through surface disinfection method. 91 endophytes were isolated, which included 38 endophytic fungi, 45 endophytic bacteria and 8 endophytic actinomycetes. Antagonistic activity tried against Fusarium oxysporum f. sp. cabense. The results showed that： the inhibitory effect is not satisfactory about endophytic fungi and endophytic actinomycetes, the antagonistic bacteria YXG2 -3 was the best, inhibition zone reached up to 20 ram; followed by BYG2 -5,BYJ5 -1 ,YXJ2 -2. the potted plant was test about 4 strains of endophytic bacteria, control effect of YXG2 -3 were 63.8%, which were highly signif- icant compared with other bacteria.