缩泉丸对自然衰老大鼠逼尿肌AC-cAMP通路的影响

Effects of Suoquan Pills on AC-cAMP Pathway in the Detrusor of Natural Aging Rats

【目的】观察自然衰老大鼠膀胱逼尿肌中腺苷酸环化酶(AC)、环磷酸腺苷(cAMP)及蛋白激酶A(PKA)的浓度变化及PKA蛋白表达,探讨缩泉丸"缩尿"的作用机理。【方法】选用老年大鼠(20月龄)50只,分为模型组,金匮肾气丸组,缩泉丸高、中、低剂量组,每组10只;青年大鼠(3月龄)10只作为空白组。采用酶联免疫吸附(ELISA)法检测逼尿肌中AC活性、cAMP及PKA的浓度变化,采用W estern b lotting检测逼尿肌PKA蛋白的表达。【结果】与模型组比较,缩泉丸组可显著提高自然衰老大鼠逼尿肌中AC活性、cAMP及PKA含量并上调PKA蛋白的表达。【结论】缩泉丸治疗肾虚多尿的作用与其能增加自然衰老大鼠逼尿肌中AC活性、cAMP及PKA含量并增加PKA蛋白的表达有关。

Objective The mechanism of Suoquan Pills（SP） in reducing urination was investigated by observing the concentration of adenylate cyclase（AC）,cyclic adenosine monophosphate（cAMP）and protein kinase（PKA） and the expression of PKA protein in the natural aging rat bladder detrusor.Methods Fifty senile rats（20 months old） were equally randomized into five groups： model group,Jinkui Shenqi Pills group,and high-,middle and low-dose SP groups.And 10 young rats（3 months old） were chosen as control.The contents of AC,cAMP and PKA were tested by using enzyme linked immunosorbent assay（ELISA）,and PKA protein expression was detected by Western blotting method.Results In comparison with the model group,SP significantly increased AC activity,cAMP and PKA contents,and PKA protein expression in the detrusor of the natural aging rats.Conclusion The therapeutic mechanism of SP for kidney-deficiency polyuria is probably related with the increase of AC activity,cAMP and PKA contents,and PKA protein expression in the detrusor of the natural aging rats.