高静水压诱导黄瓜稳定遗传变异系的分子分析(英文)

Molecular Analysis of Stable Mutagenesis in Cucumber Induced by High Hydrostatic Pressure

采用高静水压法诱变了黄瓜品种S9925-3,产生了两个稳定的可遗传变异株。采用30对随机配对引物,对该品种的原种及两个变异株进行了扩增片段长度多态性(AFLP)分析。结果表明原种与两个变异植株之间存在明显的多态性。从聚丙烯酰胺凝胶上切下6个差异明显的条带进行DNA测序,并将获得的DNA序列与NCBI(National Center for Biotechnology Information)数据库中的黄瓜DNA数据进行比对分析,确定了变异位点,找到了变异黄瓜系表型变化的可能原因。首次完成了对高静水压处理引起的黄瓜稳定遗传变异系的分子水平分析。研究结果表明,高静水压技术是一种有效的改良作物品系的新诱变工具。

Two mutant cucumber lines are obtained from the Tianjin cucumber cultivar S9925-3 after treatment with high hydrostatic pressure. DNA fingerprint analyses are performed for the two mutants and their pa- rental wild-type cucumber cultivars by AFLP （Amplified Fragments Length Polymorphism） analysis. A total of 30 randomly selected primer pairs are used for AFLP. Ten specific different bands between the mutants and the wild-type cucumber cultivars are excised from the polyacrylamide gel for DNA sequencing. Alignment of the obtained DNA sequences with NCBI （National Center for Biotechnology Information） database identifies the mutation sites in cucumber genome,which may be the reason of phenotypic changes of the mutant cucumbers. This is the first time that a study has showed at molecular level that treatment with high hydrostatic pressure results in stable mutations in cucumber cultivar. Our results indicate that high hydrostatic pressure-induced mutational breeding can be used as an effective approach for the crop improvement.