几种螯合剂对锰致小鼠肺脏和睾丸毒性的解毒作用比较

Comparison on Antidotal Effects of Chelating Agents on Lung and Testicular Toxicity in Mice Induced by Acute Exposure to Manganese

[目的]寻找理想的锰中毒解毒剂。[方法]小鼠一次性腹腔注射MnCl(2100mg MnCl2/kg)24h后分别给予L-组氨酸(L-His)、乙二胺四乙酸(EDTA)、二乙基二硫代氨基甲酸钠(DDTC)、meso-2,3-二巯基丁二酸(DMSA)及N-乙酰-L-半胱氨酸(NAC)等五种螯合剂(2mmol/kg,腹腔注射),观察小鼠肺脏、睾丸脂质过氧化物值(LPO)和组织中锰、钙、锌浓度的变化。[结果]小鼠一次性腹腔注射MnCl(2100 mg MnCl2/kg)2d后,小鼠肺脏和睾丸中LPO值分别为(2.42±0.29)和(2.74±0.33)μmol/g.prot,锰浓度分别为(2.60±0.33)和(2.41±0.68)μg/g,均明显高于对照组,差异有统计学意义(P<0.05)。分别染锰24h后腹腔注射各螯合剂,治疗24h后L-His对锰引起的肺脏、睾丸LPO值和锰浓度的升高有显著的抑制作用。[结论]L-His对锰致小鼠肺脏和睾丸毒性有较好的解毒作用,且自身毒性小,有望成为理想的锰解毒剂。

To develop new antidotes of manganese （Mn）. [ Methods ] Mice were injected intraperitoneally with MnCl2 （100 mg MnCl2/kg） 24 h before they were injected intraperitoneally with chelating agents, including L-Histidine （L-His）, ethylene diaminetetraaeetic acid（EDTA）, Dietbyldithiocarbamate （DDTC）, meso-2, 3-dimereapto suecinc acid （DMSA） and N-acetylcysteine （NAC）. The effects of those chelating agents on lipid peroxidation （LPO） and concentrations of Mn, Ca, Zn in lung were observed. [ Results ] The Mn injection significantly increased the LPO levels （2.42 ± 0.29） and （2.74 ± 0.33）μmol/（g.prot） respectively as well as the concentrations of Mn （2.60 ±0.33） and （2.41 ±0.68）μg/g wet tissue respectively in lung and testes of mice （P〈0.05） in 2 days. L-His effectively prevented the increases of above indices. [ Conclusion ] L-His is more effective than the other chelating agents in preventing changes caused by acute exposure to Mn. L-His is a promising antidote to Mn.