淫羊藿属木兰花碱分布规律及含量影响因素研究

Distribution and influencing factors of magnoflorine in Epimedium

目的:研究木兰花碱在淫羊藿属中的分布规律和影响因素。方法:超声提取,HPLC测定了淫羊藿属29个种,以及不同部位、不同生长发育时期和不同地域野生或引种的样品共80个样品。结果及结论:全部叶片样品的质量分数在0.003%～2.603%,木兰花碱含量分布具有一定的分类意义;除了巫山淫羊藿、粗毛淫羊藿和湖南淫羊藿,种内含量比较稳定;遗传因素可能是淫羊藿中的木兰花碱的含量的主要影响因素;根茎叶中木兰花碱的含量变化随叶片中的含量不同呈现不同规律类型;朝鲜淫羊藿和拟巫山淫羊藿的不同生育期含量变化表现基本一致;另外来自湖北恩施地区的几个物种存在含量异常高的类型,且异地种植后仍保持高含量,值得深入研究。

Eighty samples of Epimedium from 29 species and were determined in this study.The content of magnoflorine in leaves range between 0.003% and 2.603%.The results showed that the content of magnoflorine was quite stable within species except E.wushanense,E.acuminatum,E.hunanense.Genetic factors might be the main influencing ones.The contents of different parts and different collecting time of the medicinal materials were variable.