RP-HPLC同时测定湖北旋覆花中3种倍半萜内酯的含量

Simultaneous determination of three sesquiterpene lactones in Inula hupehensis by RP-HPLC

目的:建立RP-HPLC同时测定湖北旋覆花中锦菊素,二氢锦菊素,银胶菊素3种倍半萜内酯含量的方法。方法:Agilent C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水,线性梯度洗脱,测定波长210 nm,柱温40℃,流速1.2 mL.min-1,进样量10μL。结果:锦菊素,二氢锦菊素,银胶菊素的质量浓度分别在0.017 92～0.179 2 g·L-1(r=0.999 9),0.042 4～0.424 0 g·L-1(r=0.999 6),0.044 8～0.448 0 g·L-1(r=0.999 6)时线性关系良好,平均加样回收率依次为98.5%,98.2%,98.4%,RSD为1.3%,1.3%,1.7%。不同品种旋覆花药材中3种倍半萜内酯的含量差异较大。结论:该方法简便、快速、准确,适合于同时测定湖北旋覆花中锦菊素,二氢锦菊素,银胶菊素的含量,可用于湖北旋覆花的质量控制和旋覆花药材的真伪鉴定。

Objective： A RP-HPLC method was developed for simultaneous determination of bigelovin,ergolide and tomentosin in Inula hupehensis.Method： An Agilent C18 column（4.6 mm×250 mm,5 μm） was used for separation at 40 ℃.The mobile phase was acetonitrile-water,and the flow rate was 1.2 mL·min-1.The detection wavelength was set at 210 nm.Result： The method has good linearity in the ranges of 0.0179 2-0.179 2 g·L-1（r=0.999 9） for bigelovin,0.042 4-0.424 0 g·L-1（r =0.999 6） for ergolide,and 0.044 8-0.448 0 g·L-1（r =0.999 6） for tomentosin.The average recoveries of bigelovin,ergolide,and tomentosin were 98.5%,98.2%,98.4%,with the RSD of 1.3%,1.3%,1.7%,respectively.The results demonstrated that there was a significant difference in the contents of three sequterpene lactones among the tested Inulae Flos.Conclusion： The results indicated that the present RP-HPLC method is simple,quick and accurate,and can be used for the quality control of I.hupehensis,especially for the authentication of Inulae Flos.