发光二极管荧光显微镜在基层应用的多中心研究

Multicenter study of application of fluorescent-staining light-emitting diodes fluorescence microscope at county level in China

目的评价荧光染色发光二极管荧光显微镜涂片镜检(简称LED)在基层实验室的应用情况。方法对研究期间到6个县(区)级结核病防治机构(简称结防机构)就诊的肺结核可疑症状者3691例和随访患者2509例,以及8例没有记录初诊随访患者的共11 288份痰标本进行涂片镜检,每份标本涂2张片,分别使用萋尼(简称Z-N)和LED法进行染色、镜检,比较两种方法在阳性检出率及读片时间等方面的差异。结果 LED法的阳性涂片检出率为11.20%(1264/11 288),较Z-N法(8.59%,970/11 288)提高2.61%(χ2=8086.74,P=0.000)。读片时间LED[(120.03±38.88)s]明显短于Z-N法[(206.31±75.86)s](t=28.12,P=0.00)。结论基层实验室使用LED较Z-N能够提高涂片镜检的阳性检出率,且读片时间明显缩短,在我国基层实验室应用具有较好前景。

Objective To assess the application status of fluorescent-staining light-emitting diodes fluorescence microscope（LED） in laboratories at county level in China. Methods Two smears were prepared for each sputum from 11288 specimens coming from 3691 pulmonary tuberculosis suspects,2509 follow-up patients and 8 cases without record of diagnosis or follow-up.Each of smears were followed with Zeihl-Neelsen（Z-N） and LED respectively.The comparison of smear positive detection rate and reading time between two methods were made. Results The smear positive detection rate using LED were 11.20%（1264/11288）,2.61% higher than that of Z-N（8.59%,970/11288）（χ^2=8086.74,P=0.000）.The mean reading time with LED（120.03±38.88）s was significantly shorter than that with Z-N（206.31±75.86）s（t=28.12,P=0.00）. Conclusion LED method could increase smear positive detection rate and shorten reading time compared with Z-N,so application of LED at county level laboratories is in prospect.